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M9480120.TXT
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1994-08-09
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Document 0120
DOCN M9480120
TI Phylogenetic relationship between human immunodeficiency virus type 1
(HIV-1) long terminal repeat natural variants present in the lymph node
and peripheral blood of three HIV-1-infected individuals.
DT 9410
AU Ait-Khaled M; Emery VC; Division of Communicable Diseases, Royal Free
Hospital School of; Medicine, Hampstead, London, U.K.
SO J Gen Virol. 1994 Jul;75 ( Pt 7):1615-21. Unique Identifier : AIDSLINE
MED/94292912
AB PCR has been used to amplify a 540 base pair fragment of the human
immunodeficiency virus type 1 (HIV-1) long terminal repeat region
encompassing the U5/R/U3 regions from proviral HIV DNA that was present
in the lymph nodes and peripheral blood mononuclear cells of three
HIV-infected individuals. The resulting PCR products were cloned and the
DNA sequence of multiple clones from each reaction was determined to
enable the distribution and phylogenetic relationship between the
variants in each body site to be assessed. The results of bootstrapped
parsimony phylogenetic analyses showed that a distinct polarization was
evident between peripheral blood and lymph node variants in the patient
who possessed a histologically intact lymph node. In contrast, similar
analyses conducted on samples from two patients with extensive lymph
node disruption showed similar variants in lymph node and peripheral
blood. In the patient with an intact lymph node, lymph node variants
were significantly more heterogeneous than those present in blood
samples taken either simultaneously or 11 months later. No differences
in heterogeneity between lymph node and peripheral blood were observed
in patients with disrupted lymph nodes. The significance of these
results for our understanding of HIV pathogenesis is discussed.
DE Base Sequence Cloning, Molecular Consensus Sequence DNA,
Viral/ANALYSIS/BLOOD Human HIV Infections/*MICROBIOLOGY HIV Long
Terminal Repeat/*GENETICS HIV-1/*GENETICS Leukocytes,
Mononuclear/MICROBIOLOGY Lymph Nodes/MICROBIOLOGY Molecular Sequence
Data *Phylogeny Polymerase Chain Reaction Proviruses/GENETICS
Sequence Alignment Sequence Analysis, DNA Support, Non-U.S. Gov't
Variation (Genetics)/*GENETICS JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).